The Science Behind Bonicel

Ganeden is a science-driven organization that invests millions of dollars into ongoing research. Ganeden brings probiotic and probiotic-derived products to market only when they demonstrate safety and efficacy, providing consumers with actual benefits – not just marketing hype.

Facial Lotion Clinical Pilot Study

Ganeden performed an 8 week study to evaluate and compare the effectiveness of a topical anti-aging product containing Bacillus ferment (Bonicel) versus placebo to improve skin hydration & moisturization, improve skin elasticity, reduce the appearance of fine lines & wrinkles around the eye area, decrease the appearance of under-eye puffiness and decrease the appearance of dark circles.

The test group consisted of female subjects in the age range of 35-60 years with at least 25% having under eye puffiness and 25% having dark circles.

The inclusion of Bonicel was found to

  • Decrease the number of coarse skin lines by 20.57%
  • Show a 17% increase in the number of subjects showing improvement of eye area fine lines & wrinkles
  • Show a 8.33% increase in the number of subjects showing improvement of under eye puffiness
  • Increase skin hydration by 7.13%
  • Decrease skin shadows by 7.09%
  • Increase skin smoothness by 4.33%
  • Increase skin elasticity by 3.11%

Facial Lotion Clinical Confirmation Study

Ganeden performed a 28 day study to confirm the findings of previous studies that demonstrated the multiple benefits to using a cream with 5% Bonicel.

The study was randomized, double-blind and placebo controlled on female subjects in the age range of 35-60 with mild to moderate fines lines & wrinkles. Subjects were not using anti-aging products, nor had used them within 30 days of the start of the trial.

The subjects using the product containing Bonicel experienced:

  • Decreases in the appearance of fine lines & wrinkles of 62.9%
  • Decreases in the appearance of pore size of 48.1%
  • Decreases in the appearance of redness of 33.3%
  • Reductions in roughness of 19.4%
  • Increases in moisturization of 19.1%
  • Increases in elasticity of 5.2%

Bonicel reduces the appearance of Lines and Wrinkles
Reduction in the appearance of fine lines & wrinkles

Bonicel reduces the appearance of Pore Size
Reduction in the appearance of pore size

Hand Dryness Clinical Study

Bonicel was added at a 5% inclusion rate to a cream and was shown to decrease dryness by 89.8% in one week.

Multiple previous trials have demonstrated that when Bonicel is included at 5% there are many anti-aging benefits, including increasing facial hydration. In this study, the effects on subjects with dry and cracked hands was evaluated.

Subjects were given a cream that contained Bonicel at a 5% inclusion rate and were instructed to wash their hands twice daily for 7 days. Subjects were instructed not to use any other daily moisturizers. High-resolution photographs were taken at baseline and day 7 for analysis.

Hand dryness and cracking image 1

Hand dryness and cracking image 2

Cleansing Bar Clinical Study

Bonicel was added at a 5% inclusion rate to a cleansing bar (Bradford Soap Works) which then demonstrated a 35% reduction in facial redness in 14 days.

Three previous trials demonstrated that when Bonicel is included at 5% there are many anti-aging benefits, including reduction in redness. These three previous trials were conducted in leave on creams. This study was conducted to demonstrate that Bonicel would have positive effects, even when used in a wash off product.

Design Subjects were given a moisturizing bar that contained Bonicel at a 5% inclusion rate and were instructed to wash their face twice daily for 14 days. Subjects were instructed not to use any other daily moisturizers, and were allowed to apply normal make-up. High-resolution photographs were taken at baseline and day 14 for analysis.

Conclusion:  Bonicel at a 5% inclusion decreases redness, even in a product that is applied and washed off.

Cleansing bar image 1

Cleansing bar image 2

Gene Regulation Therapy Study

Treatment of full thickness in vitro skin cultures with 5% Bonicel produced statistically significant changes in genes related to hydration, epidermal barrier integrity and structural proteins. This supports results seen in multiple human trials, that Bonicel® at 5% inclusion, has positive effects on multiple endpoints related to skin aging.

The objective of this study was to understand how a topical material influences gene expression in the skin It was conducted using a full thickness in vitro skin model (EFT-400, MaTek). One hundred μL of test material was applied to the surface of each test culture and collected 24 hours post application. Tissues were collected in RNA later for gene expression analysis. The active group contained Bonicel® at a 5% inclusion rate, and was compared to a water control group.

Statistics were carried out using StatMiner software v4.2 (unpaired t-tests, p<0.05, N=4) to compare each treatment to the water control group. qPCR data was normalized using GAPDH as an endogenous control gene. Changes in gene expression are reported as linear fold change (FC) differences between the groups treated with test material and the control group. Positive values indicate increased gene expression; negative values indicate decreased gene expression. The statistically significant genes with FC values greater than 2.0 (increased or decreased) are shown in the Table below. Changes in excess of 2.0 are considered biologically significant.

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