Frequently Asked Questions

What is a Probiotic?

As defined by the World Health Organization (WHO), probiotics are live microorganisms, which, when administered in adequate amounts, confer a health benefit on the host.  In other words, probiotics are “good”, “friendly” or “healthy” bacteria that, when ingested at adequate levels, may help support good health. 

What does CFU stand for?

Colony Forming Units. This is the standard measurement used to quantify probiotic cell counts.

How much GanedenBC30 should we put into our product?

Dosage levels depend on the recovery rate of the material, post-processing and at the end of the product shelf life. In general, dosages range from several hundred million CFU per serving to 2 billion CFU per serving. 

Can’t I just use a generic probiotic strain in my food product? Isn’t a probiotic a probiotic?

Simply put, no.  Probiotic safety and efficacy varies from strain to strain.  While some companies simply list their probiotic by the genus and species, the real difference is in the strain.  For example, Lactobacillus acidophilus describes only the genus and species and tells nothing about the strain, its safety and its benefits.  GanedenBC30  (properly identified as Bacillus coagulans GBI-30, 6086) is a patented strain that is very different from other strains, even strains of the same genus and species.  So if you are at all concerned with documented stability, safety or efficacy, GanedenBC30 is the logical choice.  Especially when considering including a probiotic in a product that requires heating, mixing, rolling, extrusion, drying, freezing or where you require viability on the shelf with or without refrigeration.

I’ve heard that Bacillus is not well suited for use in food applications. 

Bacillus species and strains can vary dramatically. Some Bacillus strains should not be used in products meant for human or animal consumption. But GanedenBC30 (Bacillus coagulans GBI-30, 6086) is exceptionally safe, effective and stable. Billions of servings of GanedenBC30 have been consumed without a single reported adverse effect and it has never has been shown to be detrimental to food products in processing or on the shelf.

How do you clean equipment used in the processing of products made with GanedenBC30?

GanedenBC30 is very simple to clean and manage in a manufacturing environment. Unlike some probiotic strains, GanedenBC30 does not adhere to surfaces and has been validated as effectively cleaned  with a variety standard, food grade sanitizers.

Can GanedenBC30 be used in low and high pH products?

Although it depends on the application, GanedenBC30 spores have been shown to remain viable throughout a very wide pH range.

Is GanedenBC30 stable through the end of shelf life?

GanedenBC30 is stable for very long periods of time and can be found in products with shelf lives of over two years but it depends on the application, environment and storage conditions. 

Are there any common food ingredients that are not compatible with GanedenBC30?

We have not found any ingredients that affect the shelf life of GanedenBC30.

How does Ganeden work with manufacturers to ensure viability of cells?

We work with our partners through every step of manufacturing to ensure cell viability. We test samples of finished products to ensure activity levels and survival through processing and have validated third-party labs to support our clients.

What label claims can be made with GanedenBC30?

Products specific claims would require clinical testing in the finished form, but general probiotic claims may be appropriate so long as inclusion rates are appropriate and testing is completed to verify viability. GanedenBC30 has been shown to support immune health at 500 million CFUs per day and digestive health at 1 billion CFUs per day, and has been shown to survive stomach acid much more effectively than traditional probiotic strains.  

We would like to make a “kitchen sink” product with many different strains of probiotics so we can make a number of health claims. Is GanedenBC30 compatible with other strains?

We do not recommend multiple strain formulations as clinical support for probiotic cocktails are limited. Plus, GanedenBC30 is a unique strain with substantial advantages over many other strains. For example, in most standard food manufacturing processes any other strain that would be used would not remain viable after manufacturing. The only strain that would remain viable after manufacturing would be GanedenBC30 making the combination of strains worthless.

What is Bonicel

Bonicel is a patented, science-backed, probiotic-derived cosmeceutical ingredient. Bonicel has been shown to increase skin moisturization, increase skin elasticity, reduce the appearance of fine lines and wrinkles, reduce the appearance of roughness, reduce the appearance of pore size and reduce the appearance of redness. Bonicel is the trademarked name for the supernatant of GanedenBC30 (Bacillus coagulans GBI-30. 6086), Ganeden Biotech’s popular shelf-stable probiotic ingredient. Bonicel is produced during the fermentation of GanedenBC30 and is optimized to contain the perfect levels of naturally derived metabolites.  Bonicel can be formulated for use in anti-aging creams, gels, masks, serums, body lotions, cosmetics and hair care products.


What is supernatant?

Supernatant is the metabolic byproduct produced during the bacterial fermentation process.

What are probiotics?

According the FAO/WHO,  probiotics are defined as: Live microorganisms which when administered in adequate amounts confer a benefit on the host.

Is Bonicel a probiotic?

Bonicel is a probiotic-derived ingredient. It is derived from the an optimized form of GanedenBC30's fermentation broth. Although a probiotic organism produces Bonicel, Bonicel is not a probiotic.

How is Bonicel produced?

Ganeden utilizes proprietary processes to maximize the metabolic activity of the fermenting organism producing maximum amounts of:

 • Naturally derived L+ Lactic acid

 • Bacteriocins

 • Enzymes

 • Other metabolites

What does Bonicel do?

Improves skin elasticity
Improves skin moisturization
Reduces the appearance of fine lines & wrinkles
Reduces the appearance of roughness
Reduces the appearance of pore size
Reduces the appearance of redness
Reduces the appearance of under eye puffiness

Is Bonicel natural?

Bonicel is naturally derived from Ganeden’s patented, GRAS probiotic strain Bacillus coagulans GBI-30, 6086 which is marketed under the trade name, GanedenBC30.

Are there preservatives added?

Our proprietary process protects the metabolic activity of the probiotic without the use of preservatives.

What are the properties of this ingredient?

Bonicel is a water-soluble liquid, it has a light amber color and the pH range is 4.0-4.5. It incorporates easily into topical formulations. The recommended inclusion level is 5%. Bonicel is for external use only.

How do I store Bonicel?

Store at room temperature, not to exceed 70 degrees.

What is Bonicel’s INCI Name?

Bacillus ferment.

What applications can Bonicel be used in?

Bonicel can be used in any anti-aging creams, gels, masks, serums and body lotions, skin care, cosmetics and hair care products.

Is Bonicel backed by research?

Yes. Bonicel has been studied in a number of human clinical trials which:

 • Provides proof to the scientific community that Bonicel is safe and effective

 • Directly links claim support to research

 • Create strategic value and competitive advantages

 • Shows commitment to advancing science for the interest of consumers

 • Provides support for regulatory agencies

Ganeden is a science focused organization dedicated to bringing superior probiotics to markets that have been otherwise unmet by traditional solutions.

All Ganeden ingredients are made in the United States of America.