The Research


 Peer-Reviewed, Published Studies and Counting

We engage in rigorous scientific research and testing of all of its ingredients to ensure the most stable, efficacious probiotic finished products available. Here you’ll find links to published probiotic clinical studies on GanedenBC30.

Inactivated probiotic Bacillus coagulans GBI-30 induces complex immune activating, anti-inflammatory, and regenerative markers in vitro

Journal of Inflammation Research

Jensen G.S., Cash H.A., Farmer S., Keller D.


Previous studies have demonstrated that the cell walls of Bacillus coagulans GBI-30, 6086 even when inactivated can positively support the immune system. This study was demonstrated that even when the cells are inactivated through a commercial process, there is no loss of the immune function.

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The Combined Effect of Bacillus Coagulans GBI-30, 6086 and HMB Supplementation on Muscle Integrity and Cytokine Response during Intense Military Training

Journal of Applied Physiology

Yftach Gepner, Jay R. Hoffman, Elad Shemesh, Jeffrey R. Stout, David D. Church, Alyssa N. Varanoske, Hila Zelicha, Ilan Shelef, Yacov Chen, Hagai Frankel, Ishay Ostfeld


The purpose of this study was to compare the co-administration of the probiotic Bacillus coagulans GBI-30, 6086 (BC30) with β-hydroxy-β-methylbutyrate (HMB) calcium (CaHMB) to CaHMB alone on the inflammatory response and muscle integrity during 40-days of intense military training. Soldiers were randomly assigned to one of two groups: CaHMB with BC30 (CaHMBBC30; n=9) or CaHMB with placebo (CaHMBPL, n=9). A third group of participants served as a control (CTL; n=8). During the first 28 days soldiers were garrisoned on base and participated in the same training tasks. During the final 2 weeks soldiers navigated 25-30 km per night in difficult terrain carrying ~35 kg of equipment. All assessments (blood draws and diffusion tensor imaging to assess muscle integrity) were conducted prior to and approximately 12 hours following final supplement consumption. Analysis of covariance was used to analyze all blood and muscle measures. Significant attenuations were noted in IL-1β, IL-2, IL-6, CX3CL1, and TNF-α for both CaHMBBC30 and CaHMBPL compared to CTL. Plasma IL-10 concentrations were significantly attenuated for CaHMBBC30 compared to CTL only. A significant decrease in apparent diffusion coefficients was also observed for CaHMBBC30 compared to CaHMBPL. Results provide further evidence that HMB supplementation may attenuate the inflammatory response to intense training, and that the combination of the probiotic BC30 with CaHMB may be more beneficial than CaHMB alone in maintaining muscle integrity during intense military training.

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Bacillus coagulans GBI-30, 6086 increases plant protein digestion in a dynamic, computer-controlled in vitro model of the small intestine (TIM-1)

Beneficial Microbes, 2017; 8(3): 491-496

D. Keller, R. Van Dinter, H. Cash, S. Farmer and K. Venema


The aim of this study was to assess the potential of the probiotic Bacillus coagulans  GBI-30, 6086 [GanedenBC30 ] (BC30) to aid in protein digestion of alimentary plant proteins. To test this, three plant proteins, from pea, soy and rice, were digested in a validated in vitro  model of the stomach and small intestine (TIM-1) in the absence and in the presence of BC30. Samples were taken from the TIM-1 fractions that mimic uptake of amino acids by the host and analysed for α-amino nitrogen (AAN) and total nitrogen (TN). Both were increased by BC30 for all three plant proteins sources. The ratio of TN/AAN indicated that for pea protein digestion was increased by BC30, but the degree of polymerisation of the liberated small peptides and free amino acids was not changed. For soy and rice, however, BC30 showed a 2-fold reduction in the TN/AAN ratio, indicating that the liberated digestion products formed during digestion in the presence of BC30 were shorter peptides and more free amino acids, than those liberated in the absence of BC30. As BC30 increased protein digestion and uptake in the upper gastrointestinal (GI) tract, it consequently also reduced the amount of protein that would be delivered to the colon, which could there be fermented into toxic metabolites by the gut microbiota. Thus, the enhanced protein digestion by BC30 showed a dual benefit: enhanced amino acid bioavailability from plant proteins in the upper GI tract, and a healthier environment in the colon.

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Probiotic Bacillus coagulans GBI-30, 6086 reduces exercise-induced muscle damage and increases recovery

PeerJ, July 2016

Ralf Jäger, Kevin A. Shields, Ryan P. Lowery, Eduardo O. De Souza, Jeremy M. Partl, Chase Hollmer, Martin Purpura and Jacob M. Wilson


Probiotics have been reported to support healthy digestive and immune function, aid in protein absorption, and decrease inflammation. Further, a trend to increase vertical jump power has been observed following co-administration of protein and probiotics in resistance-trained subjects. However, to date the potential beneficial effect of probiotics on recovery from high intensity resistance exercise have yet to be explored. Therefore, this study examined the effect of co-administration of protein and probiotics on muscle damage, recovery and performance following a damaging exercise bout.

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Integrate genome-based assessment of safety for probiotic strains: Bacillus coagulans GBI-30, 6086 as a case study

Applied Microbiology and Biotechnology

Elisa Salvetti, Luigi Orrù, Vittorio Capozzi, Alessia Martina, Antonella Lamontanara, David Keller, Howard Cash, Giovanna E. Felis, Luigi Cattivelli, Sandra Torriani, Giuseppe Spano


Probiotics are microorganisms that confer beneficialeffects on the host; nevertheless, before being allowed for human consumption, their safety must be verified with accurate protocols. In the genomic era, such procedures should take into account the genomic-based approaches. This study aims at assessing the safety traits of Bacillus coagulans GBI-30, 6086 integrating the most updated genomics-based procedures and conventional phenotypic assays.

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Nutritional profile and cooking quality of a new functional pasta naturally enriched in phenolic acids, added with β-glucan and Bacillus coagulans GBI-30, 6086

Journal of Cereal Science, Volume 65, September 2015, Pages 260–266

Clara Fares, Valeria Menga, Alessia Martina, Nicoletta Pellegrini, Francesca Scazzina, Sandra Torriani


A new functional pasta using a durum wheat flour rich in polyphenols, with added barley β-glucan and Bacillus coagulans GBI-30, 6086 was developed.

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